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Quiz about The Wonderful World of Microbiology
Quiz about The Wonderful World of Microbiology

The Wonderful World of Microbiology Quiz


This quiz tests your knowledge of the classification, structure and metabolism of prokaryotic cells (bacteria), as well as some common lab procedures used in microbiology. It contains basic questions from a college-level Microbiology course.

A multiple-choice quiz by violin34. Estimated time: 4 mins.
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Author
violin34
Time
4 mins
Type
Multiple Choice
Quiz #
264,960
Updated
Dec 03 21
# Qns
10
Difficulty
Tough
Avg Score
6 / 10
Plays
5515
Awards
Top 5% quiz!
Last 3 plays: Guest 162 (3/10), Guest 31 (4/10), Guest 107 (5/10).
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Question 1 of 10
1. The Gram stain is used to classify cells based on differences in their ________. Hint


Question 2 of 10
2. A small ring of DNA independent from the bacterium's main genome that can be taken in and expressed by bacteria is called a(n) __________. Hint


Question 3 of 10
3. LPS is an endotoxin that is found on the outer membrane of Gram negative bacteria. LPS stands for _________. Hint


Question 4 of 10
4. Okazaki fragments are associated with which essential process? Hint


Question 5 of 10
5. One genus of bacteria that can be found in the bottom, black layer of a Winogradsky column is _____________. Hint


Question 6 of 10
6. Which of these is used as a mordant in the Gram stain? Hint


Question 7 of 10
7. Which of these is not in the domain Bacteria? Hint


Question 8 of 10
8. The genus Mycoplasma is resistant to the effects of ____________. Hint


Question 9 of 10
9. Which type of plasmid, when taken up by a bacteria, confers the ability to create a sex pilus? Hint


Question 10 of 10
10. Which is least likely to kill bacteria? Hint



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Most Recent Scores
Mar 27 2024 : Guest 162: 3/10
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quiz
Quiz Answer Key and Fun Facts
1. The Gram stain is used to classify cells based on differences in their ________.

Answer: cell wall

Gram positive bacteria have a thick peptidoglycan cell wall, and Gram negative bacteria have a thin peptidoglycan cell wall with an outer membrane. Gram negative bacteria are also more likely to be pathogenic than Gram positive bacteria.
2. A small ring of DNA independent from the bacterium's main genome that can be taken in and expressed by bacteria is called a(n) __________.

Answer: plasmid

Plasmids are very useful in genetic engineering, enabling us to insert human genes into bacteria to produce mass quantities of human proteins.
3. LPS is an endotoxin that is found on the outer membrane of Gram negative bacteria. LPS stands for _________.

Answer: lipopolysaccharide

LPS induces a fever response in the human body. Gram negative bacteria are often heat-sensitive, so the fever will help to eliminate the infection.
4. Okazaki fragments are associated with which essential process?

Answer: DNA replication

Okazaki fragments are the small segments of DNA that are formed on the lagging strand during DNA replication.
5. One genus of bacteria that can be found in the bottom, black layer of a Winogradsky column is _____________.

Answer: Desulfovibrio

The Winogradsky column is a device that models the interactions of bacteria in the environment. Desulfovibrio are sulfate-reducing anaerobic bacteria that live at the bottom of the column.
6. Which of these is used as a mordant in the Gram stain?

Answer: iodine

The mordant helps in the uptake of dyes into the cell. Crystal violet is the primary stain, alcohol is the decolorizer, and safranin is the counterstain.

Thanks lateonenight: Iodine is technically not a mordant, but a trapping agent. To be correctly classified as a mordant, a compound must be a metal, which iodine is not. However, most Gram stain procedures refer to iodine as a mordant. For more information on the difference between trapping stains and mordants go to http://stainsfile.info/StainsFile/theory/trapping.htm
7. Which of these is not in the domain Bacteria?

Answer: Methanobacterium

Methanobacterium belongs to the domain Archaea, which differs significantly from Bacteria in chemical structure and metabolism. The term "bacteria" is commonly used to refer to all prokaryotes, or single-celled organisms lacking a nucleus.
8. The genus Mycoplasma is resistant to the effects of ____________.

Answer: penicillin

Mycoplasma has no cell wall, and since penicillins disrupt cell wall synthesis, the antibiotic has no effect.
9. Which type of plasmid, when taken up by a bacteria, confers the ability to create a sex pilus?

Answer: F-plasmid

F-plasmid stands for fertility plasmid, although my biology class used a different mnemonic. :)
10. Which is least likely to kill bacteria?

Answer: extreme cold

Extreme cold, while inhibiting the growth of most bacteria, does not usually kill them. And although heat and high and low pH will kill most known species of bacteria, certain species of Archaea can live in these extreme environments.
Source: Author violin34

This quiz was reviewed by FunTrivia editor crisw before going online.
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