- Louisville was host to the first successful hand transplant in history at the Kleinert Kutz Hand Care Center in 1999.
- Lexington's J.M. Smucker Co. building (the Jif peanut butter plant) is the largest peanut butter-producing facility in the world.
- Bowling Green is home to the Fruit of the Loom company's headquarters.
- Owensboro is home to the world's largest sassafras tree, which stands over 100 feet tall.
- Covington is famous for one of the world's largest, handmade stained-glass windows (67 ft.× 24 ft.) in its St Mary's Cathedral Basilica of the Assumption.
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